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Glycolysis Pathways

on May 06, 2022

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Isolation and Purification of DNA (Bacteria, Plant and Animal Cell)

The basic procedure requires releasing DNA from cells and purifying DNA for use in experiments. DNA isolation and purification mainly includes four steps: Cell Lysis Enzymatic treatment Purification of DNA/RNA Quantification of DNA/RNA 1. Cell lysis Culture of bacterial or eukaryotic cells (freshly prepared). The cells that need to be lysed to release their components. For bacterial cell lysis, chemical required lysozyme, with EDTA and a detergent such as SDS. EDTA removes divalent cations and therefore destabilizes the outer membrane in bacteria such as E. coli and also inhibits DNA which otherwise tends to degrade DNA, while detergents solubilize lipids in the membrane. Plant and fungal cells require alternative treatments, both mechanical and enzymatic (cellulase, chitinase). Animal cells (without a cell wall) can generally be lysed by a softer treatment with a gentle treatment . detergents (such as CTAB). Complex solution of DNA, RNA, proteins, lipids and carbohydrates. Sudden ly

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Glycolysis Pathways

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